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Multi-CADD Method Screening (E. coli efflux pump) inhibitors​

Background

The client, funded by academic sources, aims to identify commercially available small molecule compounds that serve as inhibitors of efflux pump proteins in Escherichia coli.​

Question of Interest

How can in silico studies be utilized to identify commercially available compounds that inhibit fusion proteins?​

Methods

Literature selected inhibitors (AcrA:25-AcrB:59) obtained by data mining and their derivatives (AcrA:857-AcrB:3891). Followed by virtual screening, molecular docking and density functional theory (DFT) calculations.​

Results

ZINC databases of over 11Mn compounds filtered to compounds with top hits as AcrA and ArcB inhibitors of E. coli.  

Impact

  • The academic study resulted in the identification of commercially available efflux pump inhibitors (EPIs) that can be purchased.​
  • These identified EPIs contribute to the development of innovative therapeutics for the future.​

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